Skip to main content
. 2005 Mar 8;386(Pt 3):575–581. doi: 10.1042/BJ20041150

Figure 4. CLOCK associates with the E-box-rich region of the PPARα gene in vivo.

Figure 4

PCR proceeded using primer pairs that specifically detect the E-box-rich region of the PPARα gene, the 5′ flanking region of the PPARα gene that contained no E-box or E-box-like elements, and the putative CLOCK–BMAL1 target region in the first intron of DBP gene. DNA templates were either subcloned total chromatin (Input) or immunoprecipitated chromatin (mouse IgG or Anti-FLAG). Chromatin samples were prepared from NIH3T3 cells expressing FLAG-tagged CLOCK or empty FLAG vector. PCR products were electrophoresed through a non-denaturing 2% agarose gel containing ethidium bromide, and photographed. Arrowhead indicates specific shifted band for intronic E-box-rich region. Lane M, molecular mass standards.