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. 2005 Mar 8;386(Pt 3):575–581. doi: 10.1042/BJ20041150

Figure 6. Circadian expression of human PPARα mRNA in WI-38 fibroblasts.

Figure 6

At time 0 h, WI-38 fibroblasts were stimulated with Dex. After 2 h, medium was replaced with DMEM containing 5% FBS. Results were normalized by comparison with amount of GAPDH (glyceraldehyde-3-phosphate dehydrogenase). The initial value of PPARα mRNA is shown as 100%. Results are means±S.E.M. (n=3). One-way ANOVA demonstrated significant rhythmicity of PPARα mRNA levels (P<0.05). Asterisks indicate peaks of rhythmically expressed PPARα mRNA. Representative Northern blots of total RNA from fibroblasts after Dex stimulation are shown in Supplementary Figure 4 at http://www.BiochemJ.org/bj/386/bj3860575add.htm.