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. 2024 Aug 13;18:1435212. doi: 10.3389/fnins.2024.1435212

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Copyright © 2024 Giacomoni, Åkerblom, Habekost, Fiorenzano, Kajtez, Davidsson, Parmar and Björklund.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow for glial spheroid formation and transduction with BRAVE library. (A) 2D culture of hGPCs expressing canonical glial markers (GFAP, PDGFRα) by immunocytochemistry. (B,C) Confocal immunofluorescence images of (B) four cryosections from a glial spheroid expressing glial markers on the day of AAV transduction (B), with magnification (C). (D) Schematic illustrating glial spheroid formation and viral transduction with corresponding time points. (E–H) Shared sequence homology and consensus motifs for the MNM027-MNM031 (E), MNM028-MNM032 (F), MNM029-MNM033 (G), and MNM030-MNM034 (H) variants. Data information: 2D, two-dimensional; hGPCs, hESC-derived glial progenitor cells; D, day. Scale bars 50 μm.