| 8 M Urea |
Nonvolatile, chemically
reactive, limit heating, must be diluted to <2 M before trypsin
addition |
| 1–5% Sodium dodecyl
sulfate (SDS) |
Cheap,
strong denaturation
and hydrophobicity, must be removed before trypsin |
| 1-5% Sodium deoxycholate |
Hydrophobic for membrane
proteins, easy to remove due to precipiation with acid |
|
n-Dodecyl-β-maltoside |
expensive, low amounts can
be used with trypsin and LC-MS |
| Triton X-100 |
Do not use this; if samples
already have this or NP-40, proceed with protein precipitation |
