Fig. 1:

Suppressing Purkinje cell firing evoked short latency responses in multiple brain regions that are directly innervated by the PBN but not the DCN

a. Single-unit, multielectrode array recordings were made from awake, head-restrained PCP2Cre/Halo mice across several areas in the brain. 20 ms pulses of light were delivered to the posterior cerebellum.

b. Average increases in activity from recordings in the thalamus (54/87 responding neurons), amygdala (30/73), septum (37/68), and basal forebrain (18/86) are shown in blue. Decreases in activity (black) were seen in the septum (4/68) and basal forebrain (27/86). Shaded areas indicate standard error.

c. (Left) Retrograde tracer cholera toxin subunit B (CTB) labelled with different color fluorophores was unilaterally injected into the thalamus, amygdala, basal forebrain, or septum. (Right) The deep cerebellar nuclei (DCN) and parabrachial nuclei (PBN) were then examined for retrograde labelling.

d. Injection sites are shown for thalamus, amygdala, and basal forebrain done in a single animal, and for the septum in a different animal

e. Retrograde labelling in the DCN and PBN is shown for an animal with three injections sites in d.

f. Retrograde labelling in the DCN and PBN for the animal with septum injection site in d.

g.Quantification of retrograde labelling (CTB fluorescence – background fluorescence) observed following injections into the indicated regions: thalamus injections (n=9), amygdala (n=9), basal forebrain (n=4), and septal injections (n=5). For all box plots: central mark of each box is the median, the edges represent the 25^th and 75^th percentiles, and the whiskers represent the range of data.

LD: laterodorsal, VL: ventrolateral, VP: ventral posterior, CeA: central amygdala, BLA: basolateral amygdala, LS: lateral septum, MS: medial septum, BST: bed nucleus of the stria terminalis, VP: ventral pallidum, SI: substantia innominate, and DB: diagonal band of broca.