Table 1:
c-Fos Statistics
| Figure | ROIs | Animals | Average (mean) | S.E.M. | Statistics (w=ranks) |
|---|---|---|---|---|---|
| Fig 3E+F & Extended Data Fig 4D-F: | |||||
| PBN (halo PBN implant) | Contra: 16 Ipsi: 17 |
4 | 9.5 40.5 |
1.5 8.5 |
One-sided Wilcoxon rank sum p=1.44e-5; w=155.5 |
| PBN (halo DCN implant) | Contra: 24 Ipsi: 24 |
3 | 7.0 8.0 |
1.7 2.0 |
Wilcoxon rank sum p=0.6334; w=611.5 |
| PBN (wt) | 42 total (21/21 contra/ipsi) |
4 | 11 | 0.9 | Wilcoxon rank sum vs. contra PBN p=0.3928; w=1289 vs. ipsi PBN p=8.2e-7; w=965.5 vs. contra DCN p=6.83e-4; w=549.5 vs. ipsi DCN p=0.005; w=594 |
| VN (halo PBN implant) | Contra: 26 Ipsi: 45 |
4 | 2.7 2.2 |
0.6 0.3 |
One-sided Wilcoxon rank sum p = 0.5797; w = 952 |
| VN (halo DCN implant) | Contra: 18 Ipsi: 18 |
3 | 9.3 9.4 |
2.0 2.4 |
One-sided Wilcoxon rank sum p=0.75; w=322.5 |
| VN (wt) | 32 total (16/16 contra/ipsi) |
4 | 5.0 | 0.5 | Wilcoxon rank sum vs. contra PBN p=0.003; w=1135 vs. ipsi PBN p=2.8e-5; w=1650 vs. contra DCN p=0.17; w=527 vs. ipsi DCN p=0.62; w=483.5 |
| DCN (halo PBN implant) | Contra: 30 Ipsi: 40 |
4 | 1.6 1.5 |
0.5 0.3 |
One-sided Wilcoxon rank sum p = 0.1641; w = 986 |
| DCN-Interposed (halo DCN implant) | Contra: 16 Ipsi: 15 |
3 | 14.1 66.4 |
3.8 12.7 |
One-sided Wilcoxon rank sum p = 6.3e-5; w = 158.5 |
| DCN (wt) | 32 total (16/16 contra/ipsi) Contra: 12 |
4 4 |
2.5 40.2 |
0.3 6.0 |
Wilcoxon rank sum vs. contra PBN p=0.003; w=1214.5 vs. ipsi PBN p=1.5e-3; w=1440.5 vs. contra DCN p=0.006; w=517.5 vs. ipsi DCN p=3.9e-8; w=600 |
| LC (halo PBN implant) | Ipsi: 12 Contra: 20 |
3 | 41.0 57.4 |
5.3 5.1 |
One-sided Wilcoxon rank sum p=0.42; w=146 |
| LC (halo DCN implant) | Ipsi: 21 24 total |
4 | 71.1 38.5 |
6.9 2.1 |
One-sided Wilcoxon rank sum p=0.07; w=498 |
| LC (wt) | (12/12 contra/ipsi) | Wilcoxon rank sum vs. contra PBN p=0.46; w=1214.5 vs. ipsi PBN p=0.95; w=446.5 vs. contra DCN p=0.004; w=571.5 vs. ipsi DCN p=1.88e-5; w=671.5 |
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| Fig 4L+M & Extended Data Fig 9: | |||||
| Medial Septum (halo PBN implant) | Contra: 36 Ipsi: 34 |
4 | 30.6 33.3 |
2.3 2.5 |
One-sided Wilcoxon rank sum p = 0.26; w=1264 |
| Medial Septum (halo DCN implant) | Contra: 20 Ipsi: 20 |
3 | 27.6 24.2 |
3.3 2.4 |
Wilcoxon rank sum p=0.96; w=407.5 |
| Medial Septum (wildtype) | 96 total (48/48 contra/ipsi) |
4 | 32.9 | 0.9 | Wilcoxon rank sum vs. contra PBN p=0.29; w=2188 vs. ipsi PBN p=0.77; w=2171 vs. contra DCN p=0.0026; w=758.5 vs. ipsi DCN p=0.0025; w=756 |
| Lateral Septum (halo PBN implant) | Contra: 40 Ipsi: 42 |
4 | 14.3 15.3 |
1.1 1.0 |
One-sided Wilcoxon rank sum p=0.29; w=1804 |
| Lateral Septum (halo DCN implant) | Contra: 26 Ipsi: 25 |
3 | 17.4 20.3 |
2.4 3.3 |
Wilcoxon rank sum P=0.81; w=663.5 |
| Lateral Septum (wildtype) | 96 total (48/48 contra/ipsi) |
4 | 11.5 | 0.5 | Wilcoxon rank sum vs. contra PBN p=0.02; w=3168 vs. ipsi PBN p=6.8e-4; w=3597 vs. contra DCN p=0.048; w=1883.5 |
| Amygdala (halo PBN implant) | Contra: 28 Ipsi: 32 |
3 | 5.0 10.9 |
1.0 1.1 |
vs. ipsi DCN p=0.06; w=1783.5 |
| Amygdala (halo DCN implant) | Contra: 20 Ipsi: 20 |
3 | 9.1 14.1 |
1.0 2.2 |
One-sided Wilcoxon rank sum p=7.53e-6; w=562 |
| Amygdala (wildtype) | 60 total (30/30 contra/ipsi) |
4 | 4.0 | 0.5 | One-sided Wilcoxon rank sum p=0.140; w=464 Wilcoxon rank sum vs. contra PBN p=0.57; w=1308 vs. ipsi PBN p=3.1e-9; w=2207 vs. contra DCN p=8.5e-6; w=1208 |
| Basal Forebrain (halo PBN implant) | Contra: 58 Ipsi: 54 |
4 | 4.7 11.9 |
0.4 0.8 |
vs. ipsi DCN p=6.4e-6; w=1213.5 One-sided Wilcoxon rank sum p = 1.26e-12; w = 2077 |
| Basal Forebrain (halo DCN implant) | Contra: 28 Ipsi: 28 |
3 | 8.3 6.6 |
0.9 0.8 |
Wilcoxon rank sum p=0.1313; w=706 |
| Basal Forebrain (wildtype) | 48 total (24/24 contra/ipsi) Contra: 22 |
4 4 |
7.0 29.9 |
0.5 6.1 |
Wilcoxon rank sum vs. contra PBN p=0.0015; w=2605 vs. ipsi PBN p=1.95e-6; w=3489 vs. contra DCN p=0.269; w=1180.5 |
| Cingulate Cortex (halo PBN implant) | Ipsi: 24 Contra: 22 |
3 | 47.3 18.0 |
4.2 2.4 |
vs. ipsi DCN p=0.49; w=1015 One-sided Wilcoxon rank sum p = 0.0069; w = 404.5 |
| Cingulate Cortex (halo DCN implant) | Ipsi: 22 48 total |
4 | 15.5 32.4 |
2.3 1.5 |
Wilcoxon rank sum P=0.49; w=465 |
| Cingulate Cortex (wildtype) | (24/24 contra/ipsi) Contra: 25 Ipsi: 25 |
4 | 17.0 31.8 |
2.3 3.0 |
Wilcoxon rank sum vs. contra PBN p=0.99; w=2605 vs. ipsi PBN p=9.75e-7; w=3489 vs. contra DCN p=2.7e-5; w=449 vs. ipsi DCN p=1.28e-6; w=398 |
| Piriform Cortex (halo PBN implant) | Contra: 24 Ipsi: 24 |
3 | 14.0 11.5 |
1.2 1.1 |
One-sided Wilcoxon rank sum p=3.79e-4; w = 463.5 |
| Piriform Cortex (halo DCN implant) | 48 total (24/24 |
4 | 12.6 | 0.5 | Two-sided Wilcoxon rank sum p=0.134; w=515 |
| Piriform Cortex (wildtype) | contra/ipsi) Contra: 16 Ipsi: 16 |
4 | 5.9 15.3 |
0.7 2.0 |
Wilcoxon rank sum vs. contra PBN p=0.44; w=991 vs. ipsi PBN p=7.04e-8; w=1388 vs. contra DCN p=0.19; w=986 vs. ipsi DCN p=0.27; w=783.5 |
| Preoptic Hypothalamus (halo PBN implant) | Contra: 24 Ipsi: 24 |
3 | 19.0 19.1 |
2.4 3.1 |
One-sided Wilcoxon rank sum p=6.62e-5; w = 162.5 |
| Preoptic Hypothalamus (halo PBN implant) | 32 total (16/16 | 4 | 11.7 | 0.8 | Two-sided Wilcoxon rank sum p=0.6; w=614 |
| Preoptic Hypothalamus (wildtype) | contra/ipsi) Contra: 16 Ipsi: 16 |
4 | 13.3 35.1 |
2.0 4.3 |
Wilcoxon rank sum vs. contra PBN p=0.004; w=378.5 vs. ipsi PBN p=0.02; w=654 vs. contra DCN p=0.02; w=829 vs. ipsi DCN p=0.18; w=765.5 |
| Anterior & Lateral Hypothalamus (halo PBN implant) | Contra: 24 Ipsi: 24 |
3 | 17.3 15.7 |
1.5 1.4 |
One-sided Wilcoxon rank sum p=1.58e-4; w=168 |
| Anterior & Lateral Hypothalamus (halo DCN implant) | 44 total (22/22 contra/ipsi) | 4 | 9.1 | 0.6 | Two-sided Wilcoxon rank sum p=0.32; w=636.5 |
| Anterior & Lateral Hypothalamus (wildtype) | Wilcoxon rank sum vs. contra PBN p=0.11; w=584.5 vs. ipsi PBN p=1.25e-7; w=804 vs. contra DCN p4.15e-5; w=1147 vs. ipsi DCN p=6.39e-6; w=1179 |
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| Extended Data Figure 9: | |||||
| Motor Thalamus (halo PBN implant) | Contra: 18 Ipsi: 18 |
3 | 0.6 0.5 |
0.4 0.3 |
One-sided Wilcoxon rank sum p=0.63; w=339.5 |
|
Motor Thalamus
(halo DCN implant) |
Contra: 18 Ipsi: 18 |
3 | 3.8 3.8 |
1.2 0.9 |
One-sided Wilcoxon rank sum p=0.69; w=347.5 |
| Motor Thalamus (wildtype) | 48 total (24/24 contra/ipsi) Contra: 18 Ipsi: 18 |
3 3 |
0.2 4.2 1.6 |
0.1 1.0 0.4 |
Wilcoxon rank sum vs. contra PBN p=0.29; w=649 vs. ipsi PBN p=0.56; w=627.5 vs. contra DCN p=1.56e-6; w=865 vs. ipsi DCN p=1.25e-5; w=836.5 One-sided Wilcoxon rank sum p=0.99; w=261.5 |
| Red Nucleus (halo PBN implant) | Contra: 22 Ipsi: 22 |
3 | 3.1 3.1 |
0.6 0.4 |
One-sided Wilcoxon rank sum p=0.3089; w=516.5 |
|
Red Nucleus (halo DCN implant)
Red Nucleus (wildtype) |
36 total (18/18 contra/ipsi) Contra: 18 Ipsi: 18 |
3 3 |
1.8 4.5 13.3 |
0.4 1.0 2.4 |
Wilcoxon rank sum vs. contra PBN p=0.015; w=625 vs. ipsi PBN p=0.939; w=490.5 vs. contra DCN p=0.038; w=776 vs. ipsi DCN p=0.0074; w=813.5 One-sided Wilcoxon rank sum p=0.003; w=221 |
| Ventral Tegmental Area (halo PBN implant) | Contra: 18 Ipsi: 18 |
3 | 22.1 21.9 |
2.7 3.0 |
Two-sided Wilcoxon rank sum p=0.96; w=331 |
|
Ventral Tegmental Area (halo DCN implant)
Ventral Tegmental Area (wildtype) |
36 total (18/18 contra/ipsi) | 3 | 7.1 | 0.8 | Wilcoxon rank sum vs. contra PBN p=0.01; w=336 vs. ipsi PBN p=0.055; w=599.5 vs. contra DCN p=5.47e-6; w=742.5 vs. ipsi DCN p=2.56e-6; w=751 |