Table 5: RNAseq cluster localization.

The expression of the noted genes and references noted in the right-most column were used to categorize the different clusters in Figure 5. Different shading is used to indicate putative PBN clusters (blue), cerebellar clusters and clusters with fewer than 100 neurons (grey), and clusters likely outside of the PBN (white).

Clust#	# cells	Relevant markers	% in cluster w/ marker	% of PBN cells	Putative Location	Evidence/Validation
0	576	Unassigned
1	472	Foxp2	28.8%	29.4%	Parabrachial	Figure 5 immunohistochemistry Allen Brain Atlas in situ
2	342	Lmx1a	51%	21.3%	Parabrachial	Figure 5 in situ
3	295	Lamp5	42%	-	Vestibular & Tegmentum (PDTG)	Allen Brain Atlas in situ
4	248	Sox2; Crhbp	30% 24%	-	Vestibular	Allen Brain Atlas in situ
5	212	Grp Tacr1	67% 29%	13.2%	Parabrachial	Allen Brain Atlas in situ Allen Brain Atlas in situ
6	207	Dbh; TH	88% 85%	-	Locus Coeruleus	Allen Brain Atlas in situ Allen Brain Atlas in situ
7	190	Necab1 Pax5 Tacr1	78% 33.2% 23.2%	11.8%	Parabrachial	Figure 5 in situ Allen Brain Atlas in situ Allen Brain Atlas in situ
8	135	Penk; Tacr1 Pax5 Brs3	73% 48% 43% 24%	8.4%	Parabrachial	Allen Brain Atlas in situ Allen Brain Atlas in situ Figure 5 in situ Allen Brain Atlas in situ
9	132	Gabra6	70.5%	-	Cerebellar granule cell	Allen Brain Atlas in situ
10	127	Pax5	70%	7.9%	Parabrachial	Figure 5 in situ
11	127	Slc6a5 Pde3a	50% 39.4%	7.9%	Parabrachial	Herbert et al., 20001 Allen Brain Atlas in situ
12	109	Crh	57%	-	Barrington’s	Allen Brain Atlas in situ
13	101	Chat Slc5a7	86% 89%	-	PDTG Cholinergic neurons	Allen Brain Atlas in situ Allen Brain Atlas in situ
14	99	Lhx4	21.2%	-	Tegmentum (PDTG)	Allen Brain Atlas in situ
15	98	Lmx1a	58%	-	PBN	Figure 5 in situ
16	80	Kit PCP2	70%; 33%	-	Cerebellar gabaergic neurons	Allen Brain Atlas in situ Allen Brain Atlas in situ
17	72	Piezo2	99%	-	Mesencephalic Trigeminal	Florez-Pax et al., 20162
18	66	Penk Lmx1a Drd1	89% 54.5% 35%	-	PBN	Allen Brain Atlas in situ Figure 5 in situ Allen Brain Atlas in situ
19	55	Crhbp	71%	-	Vestibular	Allen Brain Atlas in situ
20	48	Col27a1	63%	-	Dorsal Cochlear	Allen Brain Atlas in situ
21	45	Eomes	40%	-	Cerebellar unipolar brush cell	Allen Brain Atlas in situ
22	25	PCP2	28%	-	Cerebellar Purkinje cell	Allen Brain Atlas in situ
23	15	Contamination (Gfap)	73%	-	Glia