Table 6: Cluster markers and their association with different behaviors.
A number of the genes identified in the RNAseq experiments of Figure 5 are known markers of the PBN. Previous studies have used the indicated cre lines to study the anatomical projections and behavioral roles of different subtypes of PBN neurons. Listed are relevant genes the cre lines used, their locations within the PBN and the observed projections in the forebrain as well as their roles in different behaviors. In some cases a single gene identifies a single cluster, whereas in other cases multiple genes correspond to multiple clusters.
| Gene | Cre-line used | Location in PBN | Observed projections in forebrain | Observed behaviors | Clusters |
|---|---|---|---|---|---|
| Foxp2 | Foxp2-IRES-Cre (Jax 030541)3 | Across PBN & concentration in anterior/lateral3,4 | Septum, basal ganglia, amygdala, basal forebrain, hypothalamus, thalamus3 | Unknown | 1, 5, 8, 11 |
| Lmx1a | Tg(Lmx1a-cre)1Kjmi4,5 | Across posterior PBN & concentration in anterior/lateral4 | Unknown | Unknown | 2 |
| Grp | None (FISH used4) | Dorsal “head” and “waist” PBN4 | Unknown | Unknown | 5 |
| Satb2 | Satb2-IRES-Cre6 (Jax 030546) | Across PBN, waist PBN4 | Cortex, amygdala, thalamus6,7 | Taste preference6 | 2 |
| Tacr1 | Tacr1-T2A-Cre-Neo8 (Allen Institute) | Across PBN, concentrated in dorsal8 | Hypothalamus, thalamus8 | Necessary and sufficient to drive pain-related behaviors8 | 5, 7, 8 |
| Penk | Penk-IRES2-Cre9 (Jax 025112) | Across PBN & concentration in anterior-lateral PBN9 | Hypothalamus9 | Place aversion, thermoregulation9 | 1, 2, 5, 8 |
| Brs3 | Brs3-resCRE:GFP10 (Jax 030540) | Across PBN, only lateral PBN described10 | Amygdala, Thalamus, Hypothalamus, VTA10 | Unknown | 8 |
| Slc6a5 (Glyt2) | None (IHC)1 | Medial PBN, Kolliker-Fuse1 | Unknown | Unknown | 11 |
| Slc17a6 (vGlut2) | Slc17a6-IRES-Cre (Jax 016963)3 | Almost all PBN neurons3,4 | Cortex, Septum, Amygdala, Basal Forebrain, Hypothalamus, Thalamus3,7 | Place aversion, thermoregulation9 | 1, 2, 5, 7, 8, 10 |
| Calca (CGRP) | Calca-Cre (Jax 033168)11,12 | Anterior + Lateral PBN11 | Cortex, Septum, Basal ganglia, Amygdala, Basal forebrain, Thalamus11 | General alarm12 | None |
| Pdyn | Pdyn-IRES-Cre (Jax 027958)3,4,9 | Lateral PBN + Pre-Coeruleus3,4,9 | Hypothalamus, Thalamus3,9 | Place aversion, thermoregulation9 | None |