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. 2024 Aug 27;19(8):e0309245. doi: 10.1371/journal.pone.0309245

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© 2024 Chang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Percentages of (A) CAR⁺ and (B) transposase⁺ (qPBase⁺) cells were analyzed by flow cytometry on days 1, 8 and 10 after nucleofection. (C) Fold change of CARiC9-20/19 CAR-T cells after 10 days of culture in G-Rex are shown. (D) Percentage of CD4 and CD8 T cells in the CAR⁺ population on day 10 after nucleofection. (E) Distributions of T_N, T_SCM, T_CM, T_EM, and T_EFF subsets in the CD4 (upper panel) and CD8 populations (lower panel). (F) Expression of exhaustion markers PD-1, TIM-3, and LAG-3 in CARiC9-20/19 CAR-T cells at 10 days post-nucleofection. (G) Expression of senescence markers KLRG-1 and CD57 in CARiC9-20/19 CAR-T cells. (A-G) Data represent mean ± SD for 9 healthy donors, n = 9. Each set of histogram plots shown in (C), (D), (E), and (F) represents one donor.