Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Schistosoma mansoni) | NMRI strain | Wellcome Sanger Institute | ||
| Sequence-based reagent | Probes used for in situ hybridisation | Molecular Instruments | Lot numbers in Supplementary file 1r | |
| Commercial assay or kit | Chromium Next GEM Single Cell 3′ Reagent Kits v3.1, 10X Genomics 4 rxns |
10X Genomics | Cat# PN-1000128 | |
| Chemical compound, drug | Native Bacillus licheniformis Protease | Creative Enzymes | Cat# NATE-0633 | |
| Chemical compound, drug | Liberase TM (Thermolysin Medium) | Roche | Cat# LIBTM-RO | |
| Commercial assay or kit | NEB Next Cell Lysis Buffer | NEB | E6428 | |
| Commercial assay or kit | NEB low input RNA kit | NEB | E6420 | |
| Commercial assay or kit | NEBNext Ultra II FS DNA Library Prep Kit for Illumina | NEB | E7805 | |
| Software, algorithm | ImageJ (Fiji) Version 2.1.0 | Fiji | https://imagej.net/Fiji | |
| Software, algorithm | R 3.6.2 | The R foundation | https://www.r-project.org/ | |
| Software, algorithm | RStudio 1.3.1056 | Rstudio | https://rstudio.com | |
| Software, algorithm | Python 3.8.5 | Python | https://www.python.org/downloads/ | |
| Software, algorithm | Cell Ranger 6.0.1 | Cell Ranger | https://www.10xgenomics.com/support/software/cell-ranger/latest | |
| Software, algorithm | Seurat 4.3.0 | Seurat | https://satijalab.org/seurat/articles/install.html | |
| Software, algorithm | kallisto bustools 0.27.3 | kallisto bustools | https://www.kallistobus.tools/ | |
| Software, algorithm | Scanpy 1.8.2 | Scanpy | https://scanpy.readthedocs.io/en/stable/ | |
| Software, algorithm | scvelo 0.2.4 | scvelo | https://scvelo.readthedocs.io/en/stable/ | |
| Software, algorithm | kallisto 0.44.0 | kallisto; Melsted et al., 2021 | https://pachterlab.github.io/kallisto/ | |
| Software, algorithm | SAM 1.0.1 | SAM; Tarashansky et al., 2019; Zanini and Tarashansky, 2022 | https://github.com/atarashansky/self-assembling-manifold | |
| Other | WormBase ParaSite Release 18 |
WormBase ParaSite | https://parasite.wormbase.org/Schistosoma_mansoni_prjea36577/Info/Index/ | Genome version 10 |
| Software, algorithm | Adobe Creative Cloud | Adobe | https://www.adobe.com |