Figure 9. ROS-sensitized cells lack NF-κB activation.
(A) HeLa cells were pre-treated with 500 nM H2O2 (lower panels) or left untreated (upper panels). After 8 h, cells were washed and culture medium was replaced. Cells then were treated with the indicated concentrations of H2O2 for 16 h, and photographed (phase-contrast, 10×). (B) HeLa cells were pre-treated with 500 nM H2O2 or left untreated (control). After 8 h, cells were washed and treated with the indicated concentrations of H2O2 for 10 min. IKKγ was immunoprecipitated, and an IKK assay was performed using GST–IκBα as substrate (upper panel). Expression of IKK was determined by immunoblotting with anti-IKKα/β antibody (lower panel).