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. 2005 Mar 22;387(Pt 1):195–202. doi: 10.1042/BJ20041625

Figure 5. Open form of Syn-1A slowed down Kv2.1 current activation more than WT Syn-1A.

Figure 5

(A) Outward K+ currents were triggered by a +70 mV pulse (250 ms) from a holding potential of −70 mV in HEK-293 cells expressing Kv2.1 with or without syntaxins. Currents shown are normalized to its peak magnitude and overlapped for comparison. Activation time constants are obtained by an exponential fit to the rising phase of the current and are summarized in (B). Results are means±S.E.M. for 13–18 cells. *, Significantly different (P<0.05) from control (Kv2.1 alone); and **, significantly different (P<0.05) from control and WT Syn-1A. (C) To show inactivation, outward K+ currents were triggered by a prolonged +70 mV pulse (12 s) from a holding potential of −70 mV in HEK-293 cells expressing Kv2.1 with or without syntaxins. Currents shown are normalized to its peak magnitude and overlapped for comparison. Inactivation time constants are obtained by an exponential fit to the decaying phase of the current, and are summarized in (D). Results are means±S.E.M. for 7–8 cells. Neither WT nor open form Syn-1A affected the rate of inactivation.