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. 2005 Mar 22;387(Pt 1):247–255. doi: 10.1042/BJ20041561

Figure 5. Effect of metal ions on fragmentation and dimerization of the copper-loaded PrP23–231 in the presence of dopamine.

Figure 5

(A) Western blot immunoassay for copper-loaded PrP23–231. Mixtures containing 50 mM Mes (pH 7.5), 10 μM dopamine and 1 μM copper-loaded PrP23–231 (containing 5.0 μM copper) were incubated without (Nil) or with one of the indicated metal ions (5 μM) at 37 °C for 30 min. As a control, copper-loaded PrP23–231 was incubated with 10 μM dopamine only (Cont). PrP was detected by SDS/PAGE, followed by immunoblotting with antibody SAF 84. Molecular masses in kDa are indicated on the left. (B) Quantification of the Western blot data. Signals of fragments of PrP at 17–19 kDa were determined using ATTO Densitograph Software Library and plotted as a percentage of the control (zero time, Cont). Data are means±S.D. (n=3).