Figure 6. Heteronuclear spectra of Vpr-(52–96).

Superimposition of the results from the echo/antiecho HSQC experiments performed at 600 MHz, 303 K and pH 2.6, at low concentration (0.03 mM) in black and at high concentration (2.0 mM) in red. The 17 labelled amino acids have been assigned and are identified on the spectrum. Arrows indicate residues with the most substantial ¹H chemical shift variations: Thr⁵⁵, Ile⁶¹, Leu⁶⁷, Leu⁶⁸, His⁷¹ and Phe⁷².