Figure 9. Dependence of Vpr-(52–96) quantum yield on peptide concentration.

The single Trp residue at position 54 of Vpr-(52–96) was excited at 295 nm. The quantum yields were determined in 30% (v/v) CH₃CN, pH 3.0 (●), and 25 mM Mes, pH 4.5 (■). Solid lines correspond to the fit of the experimental points with eqn (2).