Table 3.
Effects of late gestational nutritional plane of primiparous beef females on cotyledonary relative mRNA expression1
| Nutritional plane2 | ||||
|---|---|---|---|---|
| Item | CON | NR | SEM3 | P-value |
| Glucose transporters | ||||
| GLUT1 (SLC2A1) | 0.72 | 0.90 | 0.07 | 0.07 |
| GLUT3 (SLC2A3) | 0.71 | 0.94 | 0.07 | 0.02 |
| GLUT4 (SLC2A4) | 0.77 | 1.22 | 0.17 | 0.06 |
| GLUT5 (SLC2A5) | 0.81 | 0.83 | 0.11 | 0.89 |
| Amino acid transporters | ||||
| 4F2hc (SLC3A2) | 1.03 | 1.09 | 0.09 | 0.61 |
| CAT1 (SLC7A1) | 0.82 | 1.01 | 0.14 | 0.31 |
| LAT1 (SLC7A5) | 1.15 | 1.08 | 0.14 | 0.74 |
| LAT2 (SLC7A8) | 1.14 | 1.11 | 0.10 | 0.82 |
| SNAT2 (SLC38A2) | 1.00 | 1.32 | 0.11 | 0.05 |
| Angiogenic factors | ||||
| VEGFA | 0.99 | 1.03 | 0.11 | 0.78 |
| FLT1 (VEGFA receptor 1) | 1.04 | 1.34 | 0.14 | 0.13 |
| KDR (VEGFA receptor 2) | 1.04 | 1.32 | 0.18 | 0.30 |
| NOS3 | 0.74 | 1.07 | 0.12 | 0.07 |
| GUCY1B3 (nitric oxide receptor) | 0.83 | 1.02 | 0.10 | 0.15 |
| PAG2 | 0.75 | 0.78 | 0.12 | 0.90 |
1Target mRNA cycle threshold values were normalized to G3PDH (reference gene) with mRNA expression calculated relative to a pooled internal control using the 2−ΔΔCT method.
2Primiparous dams were individually-fed either 100% (Control; CON) or 70% (Nutrient Restricted; NR) of estimated metabolizable energy and metabolizable protein requirements for maintenance, pregnancy, and growth from day 160 of gestation to parturition.
3Standard error of the mean for CON (n = 11 or 12) and NR (n = 11).