(A) Kyte and Doolittle hydrophobicity/hydrophilicity plot analysis of rat ACAM. Two hydrophobic domains, the signal sequence (SS) and transmembrane (TM) domain, are noted. ACAM is characterized by N-terminal V-type Ig domain (V) and C2-type Ig domain (C2). (B) Dendogram of ACAM and CTX family members. (C) Exon–intron boundaries of mouse, rat and human ACAM gene. ACAM genes are localized to chromosome 8q22 in rat (NW_044096), chromosome 9 A5.1 in mouse (NW_000352) and chromosome 11q24.1 in human (NW_024769). Exons are indicated by vertical bars and exon numbers, I to VII, are also indicated. (D) Untranslated and coding sequence of ACAM cDNAs. Exon 7, encoding cytoplasmic domains and containing 3′-untranslated region of rat, mouse and human ACAM, varies in size. UTR, untranslated regions (grey boxes).