Table 2.

The repeatability tests of multiplex real-time PCR.

Templates	Concentrations (copies/μL)	Inter-assay		Intra-assay
		Cq values (mean ± SD)	CV%	Cq values (mean ± SD)	CV%
PEDV	104	29.63 ± 0.47	1.57	30.42 ± 0.18	0.58
	105	25.20 ± 0.18	0.71	25.37 ± 0.07	0.29
	106	19.79 ± 0.69	3.50	20.24 ± 0.03	0.15
B. hyodysenteriae	104	31.95 ± 0.21	0.67	32.24 ± 0.13	0.42
	105	27.33 ± 0.06	0.20	27.20 ± 0.09	0.32
	106	22.08 ± 0.31	1.40	22.30 ± 0.04	0.17
L. intracellularis	104	30.39 ± 0.19	0.62	30.66 ± 0.11	0.36
	105	25.44 ± 0.08	0.33	25.50 ± 0.19	0.74
	106	20.71 ± 0.03	0.12	20.81 ± 0.09	0.45