Figure 5.

Effect of Elesclomol on proliferation and migration of KIRC cells and regulation of FDX1. (A). Caki-1 cells viability was measured using the CCK-8 kit after treating with Elesclomol for 24, 48,72h. (B). EdU staining of Caki-1 cells. Scale bar, 200 μm; n=3; (C). Caki-1 cell EdU flourescence intensity analysis by flow cytometry. (D). The healing/closing state of Caki-1cells by the scratch assay (representative pictures from 3 repeated experiments). (E, F). FDX1 mRNA (E) and protein (F) expression level of Caki-1 cells treated with Elesclomol (500nM) for 48h. (G). The content of Fe-S cluster was tested of Caki-1 cells c treated with Elesclomol (500nM) for 48h. Experiments were repeated three times and the data are expressed as the mean ± SEM. *P < 0.05, ** P <0.01 and *** P < 0.001.