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. 2005 Apr 5;387(Pt 2):401–409. doi: 10.1042/BJ20041178

Figure 5. Cleavage assay.

Figure 5

A 0.7% (w/v) agarose gel was run in TAE buffer (40 mM Tris/acetate, 1 mM EDTA, pH 8.0), and stained with ethidium bromide. Lane 1, pVA2241 without protein; lane 2, pVA2241 with 20-fold molar excess TraA; lane 3, pVA2241 with 80-fold excess TraA; lane 4, pVA2241 with 20-fold excess TraAN246; lane 5, pVA2241 with 80-fold excess TraAN246; lane 6, MassRuler™ DNA Ladder [high range: 10, 8, 6, 5, 4 and 3 kb (MBI Fermentas, St. Leon-Rot, Germany)]; lane 7, RSF1010 with 80-fold excess TraA; lane 8, RSF1010 with 80-fold excess TraAN246; lane 9, RSF1010 without protein. oc, open circular plasmid DNA; ccc, covalently closed circular plasmid DNA.