Figure 6. Fast inhibition of DIDS-modified RyRs by DHPR C-region peptides.

(A) The gating of a single, DIDS-modified RyR during application and withdrawal of 50 μM C_S under conditions described in Figure 5(A). Baselines are shown by the broken line. (B) Inhibition of seven DIDS-modified RyRs in one bilayer subjected to application and removal of 150 μM peptides C_S1 and C_S2. The solid line labelled ‘i_max’ denotes the current when all channels are open. (C) DIDS-modified RyRs inhibited by DHPR C-region peptides. Data are normalized to P₀ values in the absence of a peptide. The control values and number of experiments are given in Table 2.