Table 1.

Median particle diameters resulting from different extracellular vesicle (EV) enrichment steps of method 1, from different EV enrichment methods, and from unprocessed plasma

EV enrichment step	Median particle diameter [nm]	Particles from 1 ml plasma [particles]	Protein from 1 ml plasma [µg]	MS Protein amount [µg]	MS sample volume [µl]
DCC	145.9–156.7	4.3 × 109–6.7 × 109	14,133.3–16,400.0	10.0	20.3–23.6
DCC and SEC	154.1–169.1	2.1 × 109–3.1 × 109	57.7–70.0	3.1–3.8	36
DCC, SEC and concentration (frozen plasma)	148.1–165.8	7.5 × 108–1.8 × 109	12.9–25.9	10.0	20.3–31.2
DCC, SEC and concentration (non-frozen plasma)	157.5–173.4	6.4 × 108–8.6 × 108	12.2–21.6	10.0	21.4–29.2
exoRNeasy midi kit	171.3–189.2	2.1 × 109–2.6 × 109	50.4–51.5	10.0	9.7–9.9
Unprocessed	100.2–130.6	1.9 × 1011–2.3 × 1012	65,600.0–86,000.0	10.0	5.5–24.4

Particle and protein amounts enriched from 1 ml plasma were calculated by considering sample volumes, protein concentrations, particle concentrations, and the initial plasma volume. Summary of protein amounts and sample volumes of MS samples