Table 2.

Effect of sodium nitrate (NaNO₃) on methane (CH₄), pH and ammonia (NH₃) after 6, 12, and 24h of incubation with rumen microbes in an in-vitro mixed batch culture system fed varying forege:concentrate (HF = 70:30, MF=50:50, and LF = 30:70) in experiment 1.

	Diet 1
	HF				MF				LF
	NaNO3, g/kg DM													p <
Item	0	7	14	28	0	7	14	28	0	7	14	28	SE	D2	L3	DxL4
6 Hr
CH4, mM	1.33	0.54	0.27	0.02	1.19	0.64	0.30	0.13	1.48	1.02	0.32	0.18	0.15	0.020	0.001	0.350
pH	5.87	6.38	6.02	6.73	5.82	6.19	6.21	6.09	5.87	5.93	6.15	6.15	0.09	0.003	0.001	0.001
NH3, mM	5.25	6.87	8.37	5.33	5.63	6.71	5.59	6.71	3.92	4.52	6.38	6.94	1.44	0.400	0.120	0.190
12 Hr
CH4, mM	1.98	1.53	0.89	0.14	2.28	1.42	1.10	0.14	1.91	1.67	1.21	0.13	0.52	0.790	0.001	0.860
pH	5.54	5.67	5.72	5.92	5.52	5.53	5.68	5.94	5.50	5.52	5.50	5.90	0.06	0.004	0.001	0.140
NH3, mM	4.92	6.47	6.35	7.26	6.57	6.52	7.80	6.68	4.57	5.17	5.30	6.97	1.18	0.110	0.190	0.750
24 Hr
CH4, mM	1.66	1.71	1.01	0.05	1.81	1.86	1.25	0.11	2.49	2.16	1.53	0.22	0.53	0.004	0.001	0.730
pH	5.21	5.30	5.35	5.57	5.21	5.25	5.28	5.52	5.12	5.18	5.23	5.40	0.04	0.001	0.001	0.290
NH3, mM	5.31	5.56	5.63	8.17	5.03	5.15	5.26	7.50	4.96	4.98	5.40	6.66	2.50	0.240	0.001	0.940

¹HF, high forage; MF, medium forage; LF, low forage.

²Diet effect.

³Treatment level effect.

⁴Diet and level interaction.