Table 4.

Effect of bromochloromethane (BCM) on methane (CH₄), pH and ammonia (NH₃) after 6, 12, and 24h of incubation with rumen microbes in an in-vitro mixed batch culture system fed varying forege:concentrate (HF = 70:30, MF = 50:50, and LF = 30:70) in experiment 2.

	Diet 1
	HF				MF				LF
	BCM, g/kg DM													p <
Item	0	0.075	0.15	0.3	0	0.075	0.15	0.3	0	0.075	0.15	0.3	SE	D2	L3	DxL4
6 Hr
CH4, mM	1.33	0.05	0.02	0.02	1.19	0.05	0.02	0.02	1.48	0.05	0.03	0.02	0.07	0.320	0.001	0.380
pH	5.87	5.80	5.87	5.85	5.82	5.83	5.87	5.87	5.87	5.85	5.85	5.90	0.02	0.340	0.050	0.300
NH3, mM	5.25	7.20	6.53	6.76	5.63	6.24	6.79	6.95	5.92	7.28	6.65	7.96	1.20	0.410	0.030	0.820
12 Hr
CH4, mM	1.98	0.02	0.01	0.01	2.28	0.03	0.01	0.01	1.91	0.01	0.01	0.01	0.25	0.770	0.001	0.960
pH	5.54	5.51	5.46	5.48	5.52	5.46	5.43	5.39	5.49	5.31	5.27	5.37	0.03	0.001	0.001	0.010
NH3, mM	4.92	6.11	5.73	6.18	6.57	5.80	5.48	6.12	4.57	4.08	4.39	4.41	1.26	0.010	0.940	0.810
24 Hr
CH4, mM	1.66	0.02	0.01	0.00	1.81	0.01	0.00	0.00	2.49	0.02	0.00	0.00	0.22	0.220	0.001	0.170
pH	5.21	5.32	5.34	5.33	5.21	5.23	5.24	5.21	5.12	5.18	5.20	5.16	0.01	0.001	0.001	0.001
NH3, mM	5.31	5.51	7.53	4.91	5.03	5.93	5.22	5.70	4.96	4.66	2.18	3.41	1.45	0.010	0.840	0.220

¹HF, high forage; MF, medium forage; LF, low forage.

²Diet effect.

³Treatment level effect.

⁴Diet and level interaction.