Table 5.

Effect of bromochloromethane (BCM) on short chain fatty acids (SCFA) after 6, 12, and 24h of incubation with rumen microbes in an in-vitro mixed batch culture system fed varying forege:concentrate (HF = 70:30, MF = 50:50, and LF = 30:70) in experiment 2.

	Diet 1
	HF				MF				LF
	BCM, g/kg DM													p <
Item	0	0.075	0.15	0.3	0	0.075	0.15	0.3	0	0.075	0.15	0.3	SE	D2	L3	DxL4
6 Hr
Total SCFA, mM	83.87	81.83	82.10	81.95	84.65	75.63	64.89	60.09	79.13	70.71	76.60	68.42	7.24	0.060	0.230	0.61
Acetate (A), mol%	66.84	62.08	62.04	62.18	64.54	58.21	60.00	60.67	61.80	59.23	61.25	62.67	0.99	0.001	0.001	0.14
Propionate (P), mol%	24.99	30.03	29.59	29.34	25.72	31.16	30.73	30.61	24.98	29.23	28.63	28.39	1.79	0.010	0.001	0.98
Butyrate, mol%	6.99	6.81	7.24	7.31	8.26	9.18	8.11	7.73	10.89	10.49	7.94	7.79	1.55	0.002	0.120	0.22
Valerate, mol%	0.96	0.86	0.93	0.98	1.09	1.23	1.22	1.17	1.46	1.11	1.35	1.03	0.20	0.001	0.260	0.09
Isoacids, mol%	0.22	0.23	0.20	0.19	0.39	0.22	-0.07	-0.18	0.76	-0.05	0.88	0.11	0.23	0.120	0.120	0.13
A:P	2.68	2.07	2.10	2.12	2.54	1.89	1.96	1.99	2.44	2.05	2.15	2.22	0.14	0.020	0.001	0.48
12 Hr
Total SCFA, mM	110.67	108.30	109.66	121.18	100.14	93.13	98.87	100.04	103.61	96.23	89.84	92.17	4.77	0.001	0.100	0.07
Acetate (A), mol%	62.85	58.77	57.63	56.76	61.45	55.53	54.96	54.77	59.04	52.41	52.31	52.14	0.89	0.001	0.001	0.87
Propionate (P), mol%	27.01	30.05	30.68	31.35	26.74	32.36	31.64	31.14	26.47	30.02	29.98	30.12	1.84	0.130	0.001	0.79
Butyrate, mol%	8.69	9.86	10.34	10.50	10.35	10.86	12.12	12.82	12.88	16.22	16.52	16.37	1.54	0.001	0.001	0.29
Valerate, mol%	1.02	1.21	1.25	1.23	1.18	1.26	1.24	1.27	1.21	1.28	1.26	1.31	0.18	0.440	0.330	0.98
Isoacids, mol%	0.42	0.11	0.10	0.16	0.29	0.00	0.04	0.00	0.39	0.07	-0.06	0.06	0.08	0.110	0.001	0.89
A:P	2.42	1.96	1.88	1.82	2.35	1.72	1.74	1.76	2.27	1.75	1.75	1.73	0.16	0.120	0.001	0.98
24 Hr
Total SCFA, mM	134.72	108.69	113.60	128.82	138.63	116.96	117.32	117.55	129.56	110.03	122.17	119.41	7.40	0.890	0.002	0.67
Acetate (A), mol%	58.33	51.42	50.99	50.56	55.27	48.07	47.02	46.33	48.54	46.66	45.27	44.60	3.03	0.001	0.001	0.50
Propionate (P), mol%	26.84	29.60	30.61	30.68	27.69	30.48	30.58	31.27	26.93	31.11	29.80	31.03	1.31	0.160	0.001	0.27
Butyrate, mol%	13.34	17.14	16.72	16.79	15.61	19.73	20.66	20.52	22.70	20.72	22.90	22.41	3.69	0.001	0.030	0.25
Valerate, mol%	1.10	1.69	1.61	1.65	1.02	1.52	1.58	1.65	1.28	1.41	1.83	1.72	0.46	0.510	0.001	0.63
Isoacids, mol%	0.39	0.15	0.07	0.33	0.41	0.20	0.16	0.22	0.55	0.09	0.20	0.24	0.10	0.890	0.001	0.75
A:P	2.18	1.74	1.67	1.65	2.00	1.57	1.53	1.48	1.82	1.49	1.52	1.43	0.06	0.001	0.001	0.76

¹HF, high forage; MF, medium forage; LF, low forage.

²Diet effect.

³Treatment level effect.

⁴Diet and level interaction.