Table 6.

Effects of treatments combining sodium nitrate (N), sodium sulfate (S), 3-nitro-1-propionate (P), and bromochloromethane (B) on methane (CH₄), pH and ammonia (NH₃) after 6, 12, and 24 h of incubation with rumen microbes in an in-vitro mixed batch culture system fed varying forege:concentrate (HF = 70:30, MF = 50:50, and LF = 30:70) in experiments 3 and 4.

	Diet 1
	HF				MF				LF					p <
Item	Control	NS2	NSP3	NSPB4	Control	NS2	NSP3	NSPB4	Control	NS2	NSP3	NSPB4	SE	T5	D6	TxD7
6 Hr
CH4, mM	1.92	0.25	0.06	-0.03	1.92	0.26	0.11	-0.03	1.89	0.25	0.14	-0.03	0.10	0.001	0.970	1.00
pH	5.84	6.12	6.11	6.06	5.84	6.11	6.10	6.09	5.90	6.14	6.07	6.13	0.02	0.001	0.170	0.32
NH3, mM	2.91	9.16	6.53	6.30	4.27	9.11	7.41	5.82	5.83	8.96	7.21	9.29	1.51	0.001	0.110	0.51
24 Hr
CH4, mM	4.50	0.23	0.16	-0.04	4.70	0.28	0.05	-0.04	4.33	0.94	0.06	-0.04	0.13	0.001	0.490	0.01
pH	5.42	5.72	5.66	5.65	5.33	5.59	5.59	5.56	5.29	5.50	5.53	5.47	0.01	0.001	0.001	0.01
NH3, mM	3.60	7.99	7.90	9.20	2.78	8.04	6.36	8.01	1.86	6.92	6.80	6.73	1.19	0.001	0.170	0.98

¹HF, high forage; MF, medium forage; LF, low forage.

[-1pt]²NS = sodium nitrate + sodium sulfate (28 and 12 g/kg DM, respectively).

[-1pt]³NSP = NS + 3-nitro-1-propionate (2.0 g/kg DM).

[-1pt]⁴NSPB = NSP + bromochloromethane (0.3 g/kg DM).

[-1pt]⁵Treatment effect.

[-1pt]⁶Diet effect.

[-1pt]⁷Treatment and diet interaction.