Table 7.

Effects of treatments combining sodium nitrate (N), sodium sulfate (S), 3-nitro-1-propionate (P), and bromochloromethane (B) on short chain fatty acids (SCFA) after 6, 12, and 24h of incubation with rumen microbes in an in-vitro mixed batch culture system fed varying forege:concentrate (HF = 70:30, MF = 50:50, and LF = 30:70) in experiments 3 and 4.

	Diet 1
	HF				MF				LF					p <
Item	Control	NS2	NSP3	NSPB4	Control	NS2	NSP3	NSPB4	Control	NS2	NSP3	NSPB4	SE	T5	D6	TxD7
6 Hr
Total SCFA, mM	68.23	69.31	65.28	36.84	60.94	65.39	57.58	35.49	55.17	56.07	61.22	43.48	7.35	0.001	0.350	0.64
Acetate (A), mol%	68.49	73.20	71.69	64.66	67.71	73.92	76.41	65.68	66.29	73.06	78.05	74.93	5.28	0.010	0.230	0.31
Propionate (P), mol%	21.70	18.74	20.04	28.99	20.00	17.96	17.82	24.33	18.34	18.38	15.76	18.98	2.38	0.010	0.020	0.53
Butyrate, mol%	7.03	5.64	5.03	2.18	9.65	5.61	4.30	6.32	11.78	5.84	4.09	3.94	2.79	0.001	0.290	0.38
Valerate, mol%	0.70	1.16	1.09	1.47	1.03	1.15	0.80	1.56	1.05	1.14	0.62	0.83	0.56	0.270	0.530	0.64
Isoacids, mol%	2.08	1.27	2.15	2.70	1.62	1.36	0.79	2.11	2.54	1.57	1.48	1.32	0.88	0.550	0.550	0.80
A:P	3.17	3.91	3.65	2.64	3.41	4.12	4.42	2.93	3.70	4.10	4.96	4.03	0.47	0.003	0.010	0.60
24 Hr
Total SCFA, mM	89.72	89.70	79.80	82.97	95.35	92.08	82.57	86.95	99.50	94.20	81.22	89.38	7.79	0.010	0.260	0.99
Acetate (A), mol%	61.41	60.69	56.35	51.48	55.65	55.49	52.57	47.76	48.12	50.87	48.83	36.76	5.16	0.001	0.001	0.80
Propionate (P), mol%	21.33	25.46	25.98	27.91	20.30	27.38	28.20	27.69	23.41	26.95	28.91	28.82	1.48	0.001	0.090	0.64
Butyrate, mol%	15.10	10.88	13.26	17.71	20.88	14.33	15.81	20.77	25.80	19.44	19.49	30.73	3.13	0.001	0.001	0.46
Valerate, mol%	1.34	1.55	1.82	2.07	1.51	1.67	1.69	2.16	1.39	1.64	1.84	2.31	0.34	0.001	0.660	0.93
Isoacids, mol%	0.83	1.41	2.60	0.84	1.65	1.12	1.73	1.63	1.28	1.09	1.08	1.38	0.68	0.260	0.520	0.15
A:P	2.91	2.38	2.18	1.88	2.74	2.03	1.88	1.73	2.19	1.89	1.70	1.34	0.28	0.001	0.001	0.89

¹HF, high forage; MF, medium forage; LF, low forage.

²NS = sodium nitrate + sodium sulfate (28 and 12 g/kg DM, respectively).

³NSP = NS + 3-nitro-1-propionate (2.0 g/kg DM).

⁴NSPB = NSP + bromochloromethane (0.3 g/kg DM).

⁵Treatment effect.

⁶Diet effect.

⁷Diet and treatment interaction.