Table 1. Effects of temperature and transport direction on rate of lutein transport by Caco-2 TC-7 monolayers.
Lutein uptake [pmol·min−1·(mg of protein)−1] was measured at 37 °C after apical micellar lutein delivery (control=100%). Lutein uptake at 4 °C, and when micellar lutein was added in the basolateral chamber, were compared with control under the same experimental conditions. Incubation time was 30 min. Results are means±S.E.M. for three assays. *P<0.05: value calculated for a given experimental condition was significantly different from that measured for the control.
| Uptake (% of control) | ||
|---|---|---|
| Lutein concentration (μM) | 4 °C | Basolateral to apical |
| 5.0 | 37.9±2.9* | 13.1±2.7* |
| 9.7 | 46.0±4.0* | Not detectable |
| 14.2 | 68.9±3.0* | 17.3±11.5* |