Fig. 4.
HOXC10 inhibition impairs KRAS-mutant lung cancer bone metastasis by inactivating the NOD1/ERK axis. a The top 5 altered pathways of the RNA-seq data are shown (n = 3). b qPCR validation of representative differentially expressed genes by HOXC10 loss in H441-BM cells. c HOXC10 knockdown reduced NOD1 and p-ERK1/2T202/Y204 expression but not p-P38Thr180/Tyr182 and p-P65Ser536. Cells transfected with the indicated siRNAs for 48 h. The level of proteins was examined by western blot assay. d The colony-formation ability analysis of cells transfected with the indicated siRNAs. The relative clonogenic viability was normalized to vehicle-treated control. e The migration ability of transfected with the indicated siRNAs for 48 h were detected by transwell assays. The migrated cells were normalized to vehicle-treated control cells. Scale bars, 50 μm. f Cells transfected with the indicated siRNAs for 48 h. The level of E-cadherin and N-cadherin was examined by western blot assay. g–j The relative protein levels (g), colony-formation ability (h), migration ability (i), Scale bars, 50 μm, and MMP9 mRNA levels (j) of indicated cells transfected with the indicated siRNAs. Data in (b, d, e, h, i, j) represent the mean ± s.e.m. of three technical replicates, representative of three independent experiments with similar results. Panels (b, d, e) were performed unpaired two-sided Student’s t test, (h, i, j) performed one-way ANOVA with Tukey’s multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant