Figure 7. Intensities of the EPR signals obtained with livers isolated from NOM- or GSNO-treated mice and with NOM or GSNO phosphate buffer solutions.

Columns correspond to the peak-to-peak heights of the two first lines of the EPR signal indicated in arbitrary units (means±S.D. out of at least three experiments). Mice were anaesthetized 7 min after i.p. injections of 0, 2.5 or 5 μmol of NOM, and 5 or 10 μmol of GSNO and then perfused. Livers were homogenized in a fresh (MGD)₂–Fe²⁺ solution. EPR measurements were made at 77 K. The inset shows in vitro experiments performed with mixtures of 10 mM (MGD)₂–Fe²⁺ complex and 1, 2, and 5 μM NOM or GSNO solution, diluted twice in phosphate buffer. Statistical significance (using Student's t test): **P<0.001; *P<0.05; NS, non-significant.