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. 2005 Apr 5;387(Pt 2):553–560. doi: 10.1042/BJ20041377

Figure 2. Time courses of cholesterol depletion by increasing concentrations of MCD.

Figure 2

Caco-2 cells were grown to confluence on Transwell filters. The monolayers were treated with 2, 5 or 10 mM MCD (MCD was added to both the apical and basolateral chambers of the Transwells) for increasing times before the cells were extracted. The cell lysates were subsequently assayed for cholesterol and protein content. The results are expressed as percentage of the control and are the means±range for two independent experiments for 2 mM MCD and means±S.E.M. for three independent experiments for 5 and 10 mM MCD; *P<0.01 and **P<0.001.