Figure 4. Recovery of TEER after repletion of cell cholesterol.

Caco-2 cell monolayers were treated with media that did or did not contain 5 mM MCD (MCD was added to both the apical and basolateral chambers of the Transwells). After 2.5 h, the media in both the apical and basolateral chambers was replaced with fresh control media or media that contained either 5 mM MCD or MCD–cholesterol complexes. TEER was then measured every hour for 5 h. The results shown are the means±S.E.M. for three independent experiments. The numbers in brackets represent the level of total cell cholesterol expressed as a percentage of the cholesterol concentration measured in control cells and are the means for three independent experiments.