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. 2005 Apr 26;387(Pt 3):573–584. doi: 10.1042/BJ20041745

Figure 6. Disruption of sustained interactions with β-arrestin-2 does not alter the pathways of agonist-induced internalization of the orexin-1 receptor.

Figure 6

N-terminally VSV-G-tagged forms of the wild-type (a) or C1 mutant (b) of the orexin-1 receptor were transfected into HEK-293T cells along with β-arrestin-2–GFP. Cells were untreated (A) or treated with sucrose (0.4 M) (B). Subsequent to addition of anti-VSV-G antibody labelled with CypHer-5, orexin A was added (0.5 μM, 30 min) and the cells visualized. The distribution of the orexin-1 receptor (i), β-arrestin-2 (ii) and a composite of these images (iii) are shown.