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. 2005 Apr 26;387(Pt 3):573–584. doi: 10.1042/BJ20041745

Figure 7. Internalization of both the wild-type orexin-1 receptor and the C1 mutant is β-arrestin-dependent.

Figure 7

(A) Wild-type (a) or β-arrestin-1 and -2 double knock-out (b) MEF cells were transiently transfected with C-terminally eYFP-tagged forms of the wild-type (WT) orexin-1 receptor or the C1 mutant (C1). Post-transfection (24 h) the cells were treated with vehicle (i) or agonist (0.5 μM orexin A, 30 min; ii), fixed and visualized. (B) β-Arrestin-2–RFP was co-transfected with eYFP-tagged forms of the wild-type or the C1 mutant of orexin-1 receptor mutant. The cells were challenged with vehicle (a) or 0.5 μM orexin A for 30 min (b). The distribution of the orexin-1 receptor (i), β-arrestin-2 (ii) and a composite of these images (iii) are shown.