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. 2005 Apr 26;387(Pt 3):591–600. doi: 10.1042/BJ20041733

Figure 3. AMP-binding property of wild-type and mutant LdAdKs.

Figure 3

AMP-binding affinity of wild-type and mutant enzymes was compared by passing each of them through AMP–agarose affinity column. Lane 1 shows the amount of protein loaded on the column, and lane 2 shows the amount that remained bound to the column after extensive washing.