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. 2005 Apr 26;387(Pt 3):649–658. doi: 10.1042/BJ20041729

Figure 5. In vivo interaction between Lsb5p and Arf3p.

Figure 5

(A) GFP–Arf3 was expressed in wild-type cells (KAY446) and in cells lacking lsb5 (KAY583). As shown, Arf3p is still able to localize to the plasma membrane in the absence of Lsb5 expression. Scale bar, 10 μm. (B) GFP–Lsb5p was expressed in wild-type cells (KAY446) and in cells lacking Arf3 (KAY659). As shown, cells lacking Arf3 are no longer able to localize GFP–Lsb5p to the cell cortex, demonstrating a role for Arf3p in organization of the membrane-trafficking endocytic machinery. (C) Cells lacking Arf3 expression are still able to localize Sac6p to punctate cortical spots, demonstrating that the actin cytoskeleton is still organized in the ΔArf3 cells. Scale bar, 10 μm.