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. 2005 Apr 26;387(Pt 3):659–667. doi: 10.1042/BJ20041596

Figure 10. Assay of Cdk1 kinase activity in FD-M clones.

Figure 10

(A) Histone H1 in vitro kinase assay was used to determine Cdk1 kinase activity in anti-Cdk1 immunoprecipitates from parental cells and Mcl-1 overexpressing clones. Results are representative of four separate experiments. (B) Whole cell extracts from FDC-P1 or FD-M clones were prepared and blots were probed with antibodies to either Cdk1 or actin as a loading control. Results are representative of at least three independent experiments. (C) Histone H1 in vitro kinase assay was used to determine Cdk1 kinase activity in anti-cyclin B1 immunoprecipitates from vector alone and Mcl-1 overexpressing clones. Results are representative of three experiments.