Figure 4. Association of cyclin B1 and Mcl-1 with Cdk1 are independent of each other.

Cytoplasmic and nuclear extracts from HL-60 cells were immunoprecipitated with antibodies to cyclin B1 or Mcl-1. The same blot was probed for the presence of cyclin B1 (upper panel) or Mcl-1 (lower panel). ‘Ab Con – no lysate’ represents a sample of beads incubated with the respective antibodies, in the absence of cell lysate. Each of the co-immunoprecipitations was repeated in at least six independent experiments with similar results.