Figure 6. Sequence of human Mcl-1 (hMcl-1) and the Mcl-1 splice variant (hMcl-1s).

In hMcl-1, peptides underlined were identified by MS. The last 19 out of 20 amino acids are hydrophobic and make up the membrane insertion domain. In hMcl-1s, alternative splicing results in an alternative C-terminus, indicated by the underlined region. Note that the IVTLISFGAFVAK peptide identified in the band corresponding to snMcl-1 (see text) does not appear in the splice variant.