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. 2005 Apr 26;387(Pt 3):659–667. doi: 10.1042/BJ20041596

Figure 8. Assay of Cdk1 kinase activity associated with Mcl-1.

Figure 8

(A) Histone H1 in vitro kinase assay was used to determine Cdk1 kinase activity in anti-cyclin B1 or anti-Mcl-1 immunoprecipitates from nuclear or cytosol extracts of HL-60 cells 9 h after release from a G1/S block. (B) Assay of anti-Mcl-1 immunoprecipitates from cytosol or nuclear extracts were performed in the absence or presence of 25 μM roscovitine, which was added to the in vitro kinase assay. (C) Cdk1 and phospho-Tyr-15 Cdk1 of samples corresponding to (A) were detected by immunoblotting. (D) Twice as much total protein as was used for (C) was immunoprecipitated with anti-Mcl-1 and blotted for Cdk1 or phospho-Tyr-15 Cdk1.