Table 1. Mutations introduced within the SOCS3 promoter or the Sp3 gene.

The wild-type sequences and the mutations introduced (underlined and in boldface) are shown: the mutations introduced within the GC-rich motif and the distal or proximal STAT3-responsive element of the SOCS3 promoter and those mutations introduced into the Sp3 gene to achieve the different lysine mutations.

Site, region or motif	Sequence
Sites
Wild-type distal STAT	5′-ttacaagaa-3′
Mutated distal STAT	5′-tcccaggaa-3′
Wild-type proximal STAT	5′-ttccaggaa-3′
Mutated proximal STAT	5′-tcccaggaa-3′
Regions
Wild-type GC-rich	5′-cggggggcggggcg-3′
Mutated GC-rich	5′-cgggggtagggtcg-3′
Motifs
Wild-type Sp3 IKEE	5′-atcaaggaagaa-3′
Mutated Sp3 IAEE (K483A)	5′-atcgcggaagaa-3′
Mutated Sp3 IDEE (K483D)	5′-atcgatgaagaa-3′