Fig. 4. Timing-controlled division of an A:B-droplet coupling with time-delay circuit.

a, b Time-lapse images of the division of A:B-droplets with changing the $c_{{\mathrm{E}}_{\mathrm{RH}}}$ or $u_{{{\mathrm{R}}^{†}}_{\mathrm{AB}i}}^0$ (i = 1, 2) (see also Supplementary Movie 1–5). Scale bars: 10 μm. a $c_{{\mathrm{E}}_{\mathrm{RH}}}$ = 1.25, 2.5, and 5.0 × 10⁻² U/µL; ${\tilde{c}}_{\mathrm{AB}}$ = 1.5. b $c_{{\mathrm{E}}_{\mathrm{RH}}}$ = 2.5 × 10⁻² U/µL; ${\tilde{c}}_{\mathrm{AB}}$ = 1.0, 1.5, and 2.0. c, d Time courses of the division ratio r_div with changing the $c_{{\mathrm{E}}_{\mathrm{RH}}}$ or $u_{{{\mathrm{R}}^{†}}_{\mathrm{AB}i}}^0$. Three repeated experiments in each condition are shown with the same color. c $c_{{\mathrm{E}}_{\mathrm{RH}}}$ = 1.25, 2.5 and 5.0 × 10⁻² U/µL; ${\tilde{c}}_{\mathrm{AB}}$ = 1.5. d $c_{{\mathrm{E}}_{\mathrm{RH}}}$ = 2.5 × 10⁻² U/µL; ${\tilde{c}}_{\mathrm{AB}}$ = 1.0, 1.5 and 2.0. All concentrations are at the final concentration of the observed samples. Source data are provided as a Source Data file.