Figure 1. Intracellular sequestration of cholesterol in glia from Npc1^−/− mice.

Cerebellar glia isolated from Npc1^+/+ (WT, A, D), Npc1^+/− (Ht, B, E) and Npc1^−/− (KO, C, F) were grown to confluency on glass coverslips in DMEM containing 10% FBS, then stained with filipin after incubation for 2 h (A–C) or 6 days (D–F) in serum-free DMEM. All photographs were taken with the same exposure time. Results are representative of three independent experiments with similar results.