Table 2. Apo A1 does not stimulate cholesterol release from Npc1^+/+, Npc1^+/− or Npc1^−/− glia.

Cerebellar glia from Npc1^+/+ (WT), Npc1^+/− (Ht) and Npc1^−/− (KO) mice were grown to confluency in DMEM containing 10% FBS, then incubated for 3 days in serum-free DMEM with or without 10 μg/ml apo A1. Medium was collected and the mass of cholesterol was determined by GLC. Results are means±S.E.M. for three independent experiments performed in duplicate.

	Concentration (μg/mg of cell protein)
Parameter	WT	Ht	KO
Cholesterol in medium without apo A1	5.44±0.36	5.73±0.32	5.91±0.27
Cholesterol in medium with apo A1	5.27±0.18	5.50±0.13	6.23±0.29