Figure 2. Reconstitution of TACE's catalytic domain secretion by co-expression of the hR473ΔPro virus with a second virus encoding hTACE's prodomain.

T. ni cells were simultaneously infected with 5 MOI of the hR473ΔPro virus and 0, 1, 2 or 10 MOI of the prodomain virus. Culture media were harvested at 24 and 48 h post-infection and analysed by SDS/PAGE followed by Western blotting. Upper panel: anti-TACE catalytic domain mouse antibody [20]; lower panel: anti-TACE prodomain antiserum (rabbit).