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. 2005 Apr 26;387(Pt 3):797–805. doi: 10.1042/BJ20041727

Figure 4. Expression of hTACE truncates and C184A variants.

Figure 4

(A) Western blot of TACE hR473 (lanes 1 and 2) and hR473/C184A (lanes 3 and 4) culture medium supernatants at 24 h (lanes 1 and 3) and 48 h (lanes 2 and 4) post-infection; M, mature TACE; I, TACE zymogen. (B) Western blot of hR473 (lanes 1 and 2) and hR473/C184A (lanes 3 and 4) cell-associated fractions obtained after protein extraction with 1% NP40. (C) Western blot of hR651 (lanes 1 and 2) and hR651/C184A (lanes 3 and 4) culture medium supernatants 24 h (lanes 1 and 3) and 48 h (lanes 2 and 4) post-infection. (D) Western blot of hR651 (lanes 1 and 2) and hR651/C184A (lanes 3 and 4) cell-associated fractions obtained after protein extraction with 1% NP40.