Table 1. Peptide substrate cleavage by recombinant hTACE forms.

hTACE activity was measured using a Dnp-labelled peptide containing the cleavage site of pro-TNFα (SPLAQAVRSSR). Dnp-labelled substrate and product were separated using reversed-phase HPLC and monitored at 350 nm.

TACE form	kcat/Km peptide (M−1·s−1)
hR473 wild-type	1.09×106±0.54×105
hR473 C184A	1.78×105±0.20×105
hR651 wild-type	1.69×105±0.18×105
hR651 C184A	9.93×104±0.54×104