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. 2024 Aug 27;14:19865. doi: 10.1038/s41598-024-68345-3

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Overview of pathways in Brassica napus–Leptosphaeria maculans pathosystem. (a) Metabolic pathway analysis (MetPA) from negative ionization mode with the MetaboAnalystR 6.0 software package. All the matched pathways are displayed as circles. The color and size of each circle are based on p-value (darker colors indicate more significant changes of metabolites in the corresponding pathway) and pathway impact value (the larger the value, the more impact), respectively. The most impacted pathways are annotated. 1: Alanine, aspartate, and glutamate metabolism, 2: Phenylalanine metabolism, 3: Tyrosine metabolism, 4: Tryptophan metabolism, 5: alpha-Linolenic acid metabolism, 6: Riboflavin metabolism, 7: Isoquinoline alkaloid biosynthesis, 8: Stilbenoid, diarylheptanoid, and gingerol biosynthesis, 9: Glyoxylate and dicarboxylate metabolism, 10: Citrate cycle (TCA cycle), 11: Indole alkaloid biosynthesis, 12: Phenylpropanoid biosynthesis, 13: Porphyrin and chlorophyll metabolism, 14: Pantothenate and CoA biosynthesis. (b) Metabolite sets enrichment overview. The horizontal bar (white-to-red) reflects increasing statistical significance.