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. 2005 Apr 26;387(Pt 3):865–870. doi: 10.1042/BJ20042044

Figure 2. Immunodetection of Aox proteins from HNE-treated mitochondria of Arabidopsis cell cultures.

Figure 2

Isolated mitochondria (100 μl) were suspended in 1.25 of ml of reaction medium and treated with 0–700 μM HNE as indicated, for 20 min at 25 °C. Mitochondria were pelleted and re-suspended in 100 μl of wash buffer (without BSA). Samples (50 μg) were separated by SDS/PAGE, transferred on to a nitrocellulose membrane and probed with an anti-Aox antibody. (A) No further treatment; (B) treated with either 200 mM diamide or 20 mM DTT for 30 min at room temperature immediately prior to separation, as indicated. The positions of the 70- and 35-kDa molecular-mass markers are indicated on the left. Numbers under the bands represent the percentage of Aox as a dimer or monomer at each concentration of HNE.