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Cells were treated with 400 μM menadione (black), 25 μM antimycin A (grey) or 88 mM H2O2 (white). MDA equivalents and HAE in 5-ml samples of cells were determined using the TBARS and HAE assay at specified times after treatment. Each time point represents the mean±S.E.M. for three separate cultures. FW, fresh weight.
